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16 exact matches found Save this search as an Alert
Laboratory protocol
1.
Member Organization : Yale
We are doing a study in marathon runners and are interested in sweat analysis for estimating fluid balance

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2.
Member Organization : NAVBO
Tissue Harvest for Protein Gels Clear arteries well in situ, and after removal rinse and flush with Ringerˇ¦s in a petri dish. Blot tissue to dry, and snap freeze in liquid N2. Tissue can be frozen at -70C indefinitely. 1. Fill cooling...

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3.
Member Organization : NAVBO
Protocol for Reverse Zymograms Separating/Reverse Zymo Gel 3.75 ml 4X Tris-Cl, pH 8.8 3.25 ml distilled water 6.50 ml Protogel (30% mix 29:1 acrylamide:bisacrylamide (3.3% C) Biorad 161-0156 1.5 ml gelatin (1% solution) 20...

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4.
Member Organization : NAVBO
In Situ Zymography protocol 1. Cut 8 um cryosections of mouse arterial tissue and place on + charged slides as shown below. This arrangement allows for assay of 2 conditions per slide 2. Keep cut slides at -80 °C until use. 3. Wash slides...

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5.
Member Organization : NAVBO
Collagen Gel Contraction Protocol 1. Culture Rat medial SMCs in DMEM supplemented with 10% FCS and 2% Pen/Strep 2. Prepare Collagen Gel as follows: 1.5 mg/ml Collagen Solution: (8 ml) Mix: 4.0 ml Vitrogen collagen (3 mg/ml...

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6.
Colleagues, I have several hundred Oragene•DNA OG-500 collection kits set to expire in May 2012. My team is simply not using them fast enough. Please let me know if you can use some. I hate to see these go to waste - I bought them at $20/each and...

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7.
I wish to study EC coupling in skeletal muscle

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8.
Member Organization : NAVBO
The pathogenic mechanisms of inflammatory bowel disease (IBD) including Crohn's disease (CD) and ulcerative colitis (UC) have yet to be elucidated despite numerous studies in gastroenterology and mucosal immunology. Dextran sulfate sodium (DSS)...

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9.
Member Organization : Cambridge
Looking for a validated protocol to extract total RNA for downstream microarray studies from human skin biopsies currently stored in 0.5mL QIAGEN RLT buffer. We have access to a fairly broad range of extraction techniques (including QIAcube,...

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10.
Member Organization : Yale
We are looking for protocols for lentiviral infection of immortalized murine endothelial cells. We already have high titre lenti particles in culture supernatants, but would like an estimation of IFU/cell needed for efficient transduction of these...

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