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Laboratory protocol
1.
Member Organization : NAVBO
Tissue Harvest for Protein Gels Clear arteries well in situ, and after removal rinse and flush with Ringer¡¦s in a petri dish. Blot tissue to dry, and snap freeze in liquid N2. Tissue can be frozen at -70C indefinitely. 1. Fill cooling...

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2.
Member Organization : NAVBO
Protocol for Reverse Zymograms Separating/Reverse Zymo Gel 3.75 ml 4X Tris-Cl, pH 8.8 3.25 ml distilled water 6.50 ml Protogel (30% mix 29:1 acrylamide:bisacrylamide (3.3% C) Biorad 161-0156 1.5 ml gelatin (1% solution) 20...

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3.
Member Organization : NAVBO
In Situ Zymography protocol 1. Cut 8 um cryosections of mouse arterial tissue and place on + charged slides as shown below. This arrangement allows for assay of 2 conditions per slide 2. Keep cut slides at -80 °C until use. 3. Wash slides...

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Research project
4.
Member Organization : Yale
Recently we demonstrated that intracellular calcium regulates proliferation of hepatic stellate cells via CaMK II. We now have preliminary evidence that CaMK II regulates matrix release and myofibroblastic differentiation of hepatic stellate cells and...

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Yale Keck Proteomics assay
5.
Member Organization : Yale
Quantitative mass spectrometry assay for this protein is available at the Yale Keck Proteomics Resource Laboratory. Please press "contact researcher" specifics regarding this assay.

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6.
Member Organization : Yale
Quantitative mass spectrometry assay for this protein is available at the Yale Keck Proteomics Resource Laboratory. Please press "contact researcher" specifics regarding this assay.

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7.
Member Organization : Yale
gi|2499843 RecName: Full=CD44 antigen; AltName: Full=Extracellular matrix receptor III; Short=ECMR-III; AltName: Full=GP90 lymphocyte homing/adhesion receptor; AltName: Full=HUTCH-I; AltName: Full=Hermes antigen; AltName: Full=Hyaluronate receptor;...

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8.
Member Organization : Yale
Quantitative mass spectrometry assay for this protein is available at the Yale Keck Proteomics Resource Laboratory. Please press "contact researcher" specifics regarding this assay.

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9.
Member Organization : Yale
Quantitative mass spectrometry assay for this protein is available at the Yale Keck Proteomics Resource Laboratory. Please press "contact researcher" specifics regarding this assay.

Contact Yale Researcher
10.
Member Organization : Yale
Quantitative mass spectrometry assay for this protein is available at the Yale Keck Proteomics Resource Laboratory. Please press "contact researcher" specifics regarding this assay.

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