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Laboratory protocol
1.
Member Organization : NAVBO
Tissue Harvest for Protein Gels Clear arteries well in situ, and after removal rinse and flush with Ringer¡¦s in a petri dish. Blot tissue to dry, and snap freeze in liquid N2. Tissue can be frozen at -70C indefinitely. 1. Fill cooling...

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2.
Member Organization : NAVBO
Protocol for Reverse Zymograms Separating/Reverse Zymo Gel 3.75 ml 4X Tris-Cl, pH 8.8 3.25 ml distilled water 6.50 ml Protogel (30% mix 29:1 acrylamide:bisacrylamide (3.3% C) Biorad 161-0156 1.5 ml gelatin (1% solution) 20...

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3.
Member Organization : NAVBO
Collagen Gel Contraction Protocol 1. Culture Rat medial SMCs in DMEM supplemented with 10% FCS and 2% Pen/Strep 2. Prepare Collagen Gel as follows: 1.5 mg/ml Collagen Solution: (8 ml) Mix: 4.0 ml Vitrogen collagen (3 mg/ml...

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Research project
4.
Member Organization : NAVBO
This approach, which is more generally applicable, allowed detection of a transcriptional signature of primitive cells in adult peripheral blood, in relationship to age, vascular stiffness and blood pressure: http://dx.plos.org/10.1371/journal.pone...

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Human Samples
5.
We are interested in studying alterations in people with lone atrial fibrillation. We need atrial tissue from normal individuals (or at least ones with out atrial fibrillation)

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Researcher's Website
Transgenic/KO animals
6.
Member Organization : NAVBO
These mice are available on a collaborative basis following execution of a simple MTA: Genesis. 2011 May 9. doi: 10.1002/dvg.20769. [Epub ahead of print] Characterization of pdgfrb-cre transgenic mice reveals reduction of ROSA26 reporter activity...

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Researcher's Website
7.
Member Organization : UCSD
PNMT promoter linked to Cre in C57BL/6 mouse

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Laboratory protocol
8.
Member Organization : Yale
My name is Yibing Qyang. I am a faculty in Yale Cardiology and Yale Stem Cell Center. We rountlinely derive doulbe, triple or knock-out ES cells from the blastcyst inner cell mass. If you have organ-specific cre, you can breed it with Rosa-YFP and get...

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Data
9.
Idiopathic pulmonary arterial hypertension (IPAH) is associated with lower levels of the pulmonary vasodilator nitric oxide (NO) and its biochemical reaction products (NO2-, NO3-) in part due to reduction of pulmonary endothelial NO synthesis. However...

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